Photoaffinity labeling of avermectin binding sites from Caenorhabditis elegans and Drosophila melanogaster.
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چکیده
منابع مشابه
Photoaffinity labeling of avermectin binding sites from Caenorhabditis elegans and Drosophila melanogaster.
An azido-avermectin analog [4'' alpha-(4-azidosalicylamido-epsilon-caproylamido-beta-alan ylamido)-4''-deoxyavermectin B1a; azido-AVM] was synthesized and used to photoaffinity label avermectin binding sites present in the membranes of Caenorhabditis elegans and Drosophila melanogaster. Azido-AVM was biologically active and behaved like a competitive inhibitor of [3H]ivermectin binding to C. el...
متن کاملImmunoaffinity purification of avermectin-binding proteins from the free-living nematode Caenorhabditis elegans and the fruitfly Drosophila melanogaster.
Avermectin-binding proteins from the free-living nematode worm Caenorhabditis elegans and from the fruitfly Drosophila melanogaster were purified to homogeneity via a three-step procedure. The binding proteins were covalently labelled using a radioactive photoaffinity probe and then partially purified on a Sephacryl S-300 gel-filtration column. The radiolabelled binding proteins were then purif...
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The known binding of a derivative of the COOH-terminal tetrapeptide of gastrin with high biological activity to bovine serum albumin suggests a convenient model system for testing the affinity labeling of hormone binding sites. Photolysis of 4-azidobenzoylpentagastrin, Z-nitro-5-azidobenzoylpentagastrin, 4-acetylbenzoylpentagastrin, and 4-benzoylbenzoylpentagastrin in the presence of bovine ser...
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Vogel et al. describes a bioinformatic analysis of immunoglobulin superfamily (IgSF) members in Caenorhabditis elegans and Drosophila melanogaster (Vogel et al., 2003). We have previously published reports presenting genome-sequence-driven analyses of worm and fly IgSF members (Hutter et al. or their content is essentially ignored (Hutter et al., 2000), although they cover much of the same grou...
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The ability to form selective cell-cell adhesions is an essential property of metazoan cells. Members of the cadherin superfamily are important regulators of this process in both vertebrates and invertebrates. With the advent of genome sequencing projects, determination of the full repertoire of cadherins available to an organism is possible and here we present the identification and analysis o...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1992
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.89.9.4168